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פיתוח מודל קוליטיס מונחה Antigen ללמוד מצגת של אנטיגנים על ידי אנטיגן הצגת תאים לתאי T
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Immunology and Infection
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JoVE Journal Immunology and Infection
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
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פיתוח מודל קוליטיס מונחה Antigen ללמוד מצגת של אנטיגנים על ידי אנטיגן הצגת תאים לתאי T

עברית

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8,880 Views

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06:57 min

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September 18, 2016

DOI:

10.3791/54421-v

DOI:
10.3791/54421-v

•

06:57 min
September 18, 2016

•

6 Views
, , ,
1APC Microbiome Institute,University College Cork, 2Division of Gastroenterology and Hepatology,University Hospital Basel, 3Institute of Microbiology and Biotechnology,University of Ulm

Summary

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In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

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Cite this Article

Rossini, V., Radulovic, K., Riedel, C. U., Niess, J. H. Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells. J. Vis. Exp. (115), e54421, doi:10.3791/54421 (2016).

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