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Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
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JoVE Journal 免疫学と感染
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

DOI:
10.3791/54421-v

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06:57 min

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September 18, 2016

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, , ,
1APC Microbiome Institute,University College Cork, 2Division of Gastroenterology and Hepatology,University Hospital Basel, 3Institute of Microbiology and Biotechnology,University of Ulm

章

  • 00:05標題
  • 01:28Colonic Lamina propria Cell Isolation
  • 03:58Results: Host CX3CR1+ Macrophages Communicate with Donor OT-II Cells During OVA-driven Transfer Colitis
  • 06:03Conclusion

概要

自動翻訳

自動翻訳

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

タグ

Antigen-driven ColitisAntigen Presenting CellsT CellsInflammatory Bowel DiseaseLamina PropriaT Cell Transfer Colitis ModelMononuclear PhagocytesCD4 T CellsEpithelial Cell Removal

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