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可控合成和高度统一聚荧光追踪（<em>ñ</em> -isopropylacrylamide）微凝胶
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JoVE Journal Chemistry
Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
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可控合成和高度统一聚荧光追踪（<em>ñ</em> -isopropylacrylamide）微凝胶

DOI:
10.3791/54419-v

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11:34 min

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September 08, 2016

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, , , ,
1Institute of Physical Chemistry,RWTH Aachen University

Chapters

  • 00:05Title
  • 01:24Microgel Synthesis: Conventional Batch Synthesis of Poly(N-isopropylacrylamide) (PNIPAM) Matrix Microgels
  • 03:14Microgel Synthesis: Non-stirred Synthesis of Fluorecently Labled Poly(N-isopropylacrylamide) (PNIPAM) Microgels
  • 05:40Light Scattering Characterization
  • 07:00Particle Tracking by Wide-field Fluorescence Microscopy
  • 08:51Results: Dynamic Light Scattering Characterization and Fluorescence Tracking of Microgels in Concentrated Dispersions
  • 10:29Conclusion

Summary

Automatic Translation

Automatic Translation

非搅拌沉淀聚合提供了一种快速，可重复的成型方法来刺激敏感聚（N- -isopropylacrylamide）粒径分布窄的微粒凝胶的合成。在这个协议中合成，光散射表征和在宽视场显微镜设置这些微粒凝胶的单个粒子荧光跟踪被证明。

Tags

Poly(N-isopropylacrylamide) MicrogelsFluorescence TrackingControlled SynthesisSoft Colloidal ParticlesDynamic BehaviorGlass TransitionWide Field Fluorescence MicroscopyNon-stirred Precipitation PolymerizationDynamic Light-scatteringNIPAMBISKPSBuchner FunnelCentrifugation

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