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Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
 

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Article DOI: 10.3791/54419-v • 11:34 min • September 8th, 2016
September 8th, 2016 •
1, 1, 1, 1, 1
1Institute of Physical Chemistry, RWTH Aachen University

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résumé
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Non-stirred precipitation polymerization provides a rapid, reproducible prototyping approach to the synthesis of stimuli-sensitive poly(N-isopropylacrylamide) microgels of narrow size distribution. In this protocol synthesis, light scattering characterization and single particle fluorescence tracking of these microgels in a wide-field microscopy setup are demonstrated.

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Keywords: Poly(N-isopropylacrylamide) Microgels Fluorescence Tracking Controlled Synthesis Soft Colloidal Particles Dynamic Behavior Glass Transition Wide Field Fluorescence Microscopy Non-stirred Precipitation Polymerization Dynamic Light-scattering NIPAM BIS KPS Buchner Funnel Centrifugation
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