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Analisi trascrizionale dal nascente RNA FISH di<em> In Vivo</em> Cellule del trofoblasto giganti o<em> In Vitro</em> Culture a breve termine di Ectoplacental Cono espianti
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August 31, 2016
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Trophoblast giant cells (TGCs) play a key role in the placenta to ensure a healthy pregnancy. We present a protocol for assessing the transcriptional status of genes in TGCs by nascent fluorescent in situ hybridization on cryostat sections of post-implantation embryos or short-term cultures of embryonic day 7 ectoplacental cones.
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Transcriptional AnalysisNascent RNA FISHTrophoblast Giant CellsEctoplacental Cone ExplantsIn VivoIn VitroSingle Cell AnalysisGene ExpressionX Chromosome InactivationImprintingEmbryo DissectionEPC IsolationCell CultureCell FixationCell PermeabilizationFluorescent Probe PrecipitationEthanol Precipitation
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Corbel, C., Heard, E. Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants. J. Vis. Exp. (114), e54386, doi:10.3791/54386 (2016).
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