/
/
Transkriptions-Analyse von RNA Nascent FISH<em> In Vivo</em> Trophoblast-Riesenzellen oder<em> In Vitro</em> Kurzfristige Kulturen von Ectoplacental Cone Explantate
This content is Free Access.
7,628 Views
•
08:26 min
•
August 31, 2016
DOI:
Summary
Automatically generated
Trophoblast giant cells (TGCs) play a key role in the placenta to ensure a healthy pregnancy. We present a protocol for assessing the transcriptional status of genes in TGCs by nascent fluorescent in situ hybridization on cryostat sections of post-implantation embryos or short-term cultures of embryonic day 7 ectoplacental cones.
Explore More Videos
Transcriptional AnalysisNascent RNA FISHTrophoblast Giant CellsEctoplacental Cone ExplantsIn VivoIn VitroSingle Cell AnalysisGene ExpressionX Chromosome InactivationImprintingEmbryo DissectionEPC IsolationCell CultureCell FixationCell PermeabilizationFluorescent Probe PrecipitationEthanol Precipitation
Read Article
Cite this Article
Corbel, C., Heard, E. Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants. J. Vis. Exp. (114), e54386, doi:10.3791/54386 (2016).
Copy