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High-throughput Gene Tagging in Trypanosoma brucei
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August 12, 2016
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Addition of a tag to a protein is a powerful way of gaining insight into its function. Here, we describe a protocol to endogenously tag hundreds of Trypanosoma brucei proteins in parallel such that genome scale tagging is achievable.
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Dyer, P., Dean, S., Sunter, J. High-throughput Gene Tagging in Trypanosoma brucei. J. Vis. Exp. (114), e54342, doi:10.3791/54342 (2016).
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