ジャーナル
/
発生生物学
/
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

DOI:
10.3791/54329-v

•

09:05 min

•

August 20, 2016

•

, , , , , ,
1Department of Ophthalmology,Far Eastern Memorial Hospital, 2Institute of Clinical Medicine,National Taiwan University, 3Department of Ophthalmology,National Taiwan University Hospital, 4College of Medicine,National Taiwan University

章

  • 00:05標題
  • 01:06Primary Preparation and Immunostaining of Bovine Corneal Endothelial Cells
  • 04:24Rat Corneal Endothelium Cryoinjury Model and Intracameral Injection
  • 06:46Results: Immunostaining of In Vitro Cultured Bovine CECs with Marimastat Rat Corneal Buttons After Cryoinjury
  • 08:27Conclusion

概要

自動翻訳

自動翻訳

A primary culture of bovine corneal endothelial cells was used to investigate the mechanism of corneal endothelial-mesenchymal transition. Furthermore, a rat corneal endothelium cryoinjury model was used to demonstrate corneal endothelial-mesenchymal transition in vivo.

タグ

Corneal Endothelial-mesenchymal TransitionIn VitroIn VivoCorneal EndotheliumRegenerationBovine EyesDescemet’s MembraneCorneal Endothelial CellsSHEMTrypsinHemacytometerMaramastatParaformaldehydeTriton X-100BSAABCSnailSlug

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Live Imaging of Mouse Secondary Palate Fusion

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

Read Article