Journal
/
Genetics
/
Indagine basata RNA interferenza della funzione di shock termico proteine ​​27 durante la guarigione della ferita corneale epiteliale
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing
Please note that all translations are automatically generated. Click here for the English version.

Indagine basata RNA interferenza della funzione di shock termico proteine ​​27 durante la guarigione della ferita corneale epiteliale

italiano

Automatically Generated

7,355 Views

•

08:34 min

•

September 27, 2016

DOI:

10.3791/54280-v

DOI:
10.3791/54280-v

•

08:34 min
September 27, 2016

•

4 Views
, , , , ,
1Department of Ophthalmology,Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine,Asan Medical Center

Summary

Automatically generated

Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.

Explore More Videos

RNA InterferenceHSP27Corneal Epithelial Wound HealingSiRNATransfectionWestern BlotCell CultureProtein ExtractionProtein Quantification

Read Article

Cite this Article

Yoo, A., Park, H., Kang, S., Kim, E., Tchah, H., Kim, J. Y. RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing. J. Vis. Exp. (115), e54280, doi:10.3791/54280 (2016).

Download .ris file

Copy

Share Video

.

Copy