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角膜上皮伤口愈合过程中的热休克蛋白27的功能为基础的RNA干扰研究
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Genetics
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JoVE Journal Genetics
RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing
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角膜上皮伤口愈合过程中的热休克蛋白27的功能为基础的RNA干扰研究

English

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7,348 Views

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08:34 min

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September 27, 2016

DOI:

10.3791/54280-v

DOI:
10.3791/54280-v

•

08:34 min
September 27, 2016

•

4 Views
, , , , ,
1Department of Ophthalmology,Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine,Asan Medical Center

Summary

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Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.

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Yoo, A., Park, H., Kang, S., Kim, E., Tchah, H., Kim, J. Y. RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing. J. Vis. Exp. (115), e54280, doi:10.3791/54280 (2016).

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