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Isolement de périvasculaire Multipotentes Précurseurs cellulaires Populations de Cardiac Human Tissue
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
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Isolement de périvasculaire Multipotentes Précurseurs cellulaires Populations de Cardiac Human Tissue

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7,253 Views

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08:15 min

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October 08, 2016

DOI:

10.3791/54252-v

DOI:
10.3791/54252-v

•

08:15 min
October 08, 2016

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1 Views
, , , , , ,
1Centre for Cardiovascular Science, Queen’s Medical Research Institute,University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery,University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering,Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine,University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery,University of Pittsburgh, 6Department of Orthopaedic Surgery,University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine,University of California at Los Angeles

Summary

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du tissu cardiaque humain héberge des populations de cellules précurseurs pluripotentes périvasculaires qui peuvent être appropriés pour la régénération du myocarde. La technique décrite ici permet l'isolement et la purification simultanée de deux populations de cellules stromales pluripotentes associées aux vaisseaux sanguins d' origine, c. -à- CD146 + CD34 - péricytes et les cellules CD34 + CD146 - cellules de l' adventice, du myocarde humain.

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Cite this Article

Baily, J. E., Chen, W. C., Khan, N., Murray, I. R., González Galofre, Z. N., Huard, J., Péault, B. Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue. J. Vis. Exp. (116), e54252, doi:10.3791/54252 (2016).

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