このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
7,252 Views
•
08:15 min
•
October 08, 2016
DOI:
1Centre for Cardiovascular Science, Queen’s Medical Research Institute,University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery,University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering,Massachusetts Institute of Technology, 4MRC再生医学センター,University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery,University of Pittsburgh, 6Department of Orthopaedic Surgery,University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine,University of California at Los Angeles
概要
Automatically generated
Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium.
Read Article
Cite this Article
Baily, J. E., Chen, W. C., Khan, N., Murray, I. R., González Galofre, Z. N., Huard, J., Péault, B. Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue. J. Vis. Exp. (116), e54252, doi:10.3791/54252 (2016).
Copy