Journal
/
/
La cultura de los macrófagos derivados de monocitos-macrófagos factor estimulante de colonia humana diferenciada
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

La cultura de los macrófagos derivados de monocitos-macrófagos factor estimulante de colonia humana diferenciada

28,150 Views

06:46 min

June 30, 2016

DOI:

06:46 min
June 30, 2016

48 Views
,

Summary

Automatically generated

A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.

Read Article