/
/
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
28,241 Views
•
06:46 min
•
June 30, 2016
DOI:
Özet
Automatically generated
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
Read Article
Cite this Article
Jin, X., Kruth, H. S. Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages. J. Vis. Exp. (112), e54244, doi:10.3791/54244 (2016).
Copy