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Applicando Fluorescence Resonance Energy Transfer (FRET) per esaminare Effector Translocation efficienza da<em> Burnetii Coxiella</em> Durante siRNA Silencing
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Immunology and Infection
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JoVE Journal Immunology and Infection
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
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Applicando Fluorescence Resonance Energy Transfer (FRET) per esaminare Effector Translocation efficienza da<em> Burnetii Coxiella</em> Durante siRNA Silencing

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10,527 Views

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10:29 min

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July 06, 2016

DOI:

10.3791/54210-v

DOI:
10.3791/54210-v

•

10:29 min
July 06, 2016

•

1 Views
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1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,University of Melbourne

Summary

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Indagare le interazioni tra batteri patogeni ei loro ospiti è un'area importante della ricerca biologica. Qui, descriviamo le tecniche necessarie per misurare effettrici traslocazione da Coxiella burnetii durante siRNA silenziamento genico mediante substrato Blam.

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FRETEffector TranslocationSiRNA SilencingCoxiella BurnetiiCellular MicrobiologyHost-pathogen InteractionsChlamydiaSalmonellaE. ColiBeta-lactamaseACCM2HeLa CellsTransfection96-well Plate

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Newton, P., Latomanski, E. A., Newton, H. J. Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing. J. Vis. Exp. (113), e54210, doi:10.3791/54210 (2016).

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