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Anwenden von Fluorescence Resonance Energy Transfer (FRET) zu untersuchen Effektor Translokation Effizienz durch<em> Coxiella burnetii</em> Während siRNA Silencing
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Immunology and Infection
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JoVE Journal Immunology and Infection
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
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Anwenden von Fluorescence Resonance Energy Transfer (FRET) zu untersuchen Effektor Translokation Effizienz durch<em> Coxiella burnetii</em> Während siRNA Silencing

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10,527 Views

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10:29 min

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July 06, 2016

DOI:

10.3791/54210-v

DOI:
10.3791/54210-v

•

10:29 min
July 06, 2016

•

1 Views
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1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,University of Melbourne

Summary

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Untersuchung der Wechselwirkungen zwischen bakteriellen Erregern und ihren Wirten ist ein wichtiger Bereich der biologischen Forschung. Hier beschreiben wir die notwendigen Techniken Effektor - Translokation durch Coxiella burnetii während siRNA Gen - Silencing mit BlaM Substrat zu messen.

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FRETEffector TranslocationSiRNA SilencingCoxiella BurnetiiCellular MicrobiologyHost-pathogen InteractionsChlamydiaSalmonellaE. ColiBeta-lactamaseACCM2HeLa CellsTransfection96-well Plate

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Newton, P., Latomanski, E. A., Newton, H. J. Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing. J. Vis. Exp. (113), e54210, doi:10.3791/54210 (2016).

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