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免疫与感染
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Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

DOI:
10.3791/54128-v

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06:09 min

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June 22, 2016

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, , ,
1Translational Immunology, Institute of Molecular and Cell Biology,Agency for Science, Technology and Research, 2Singapore Immunology Network, 3Institute of Biomedical Studies,Baylor University

Chapters

  • 00:05Title
  • 00:37Monocyte Enrichment by Magnetic Separation and Magnetic Labeling
  • 01:35Magnetic Separation
  • 02:44Generating Different moDCs
  • 04:17Results: Enhanced Characterization and Analysis of moDCs
  • 05:39Conclusion

Summary

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Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.

Tags

Dendritic CellsMonocyte EnrichmentCD14+ CellsImmature Dendritic CellsMature Dendritic CellsTolerogenic Dendritic CellsMetabolic PhenotypesCell CultureIn VitroImmunologyVaccine Development

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