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Usando folha de luz microscopia de fluorescência para Imagem Desenvolvimento Zebrafish Eye
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Usando folha de luz microscopia de fluorescência para Imagem Desenvolvimento Zebrafish Eye

português

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33,385 Views

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13:01 min

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April 10, 2016

DOI:

10.3791/53966-v

DOI:
10.3791/53966-v

•

13:01 min
April 10, 2016

•

23 Views
, , , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Summary

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Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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Cite this Article

Icha, J., Schmied, C., Sidhaye, J., Tomancak, P., Preibisch, S., Norden, C. Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development. J. Vis. Exp. (110), e53966, doi:10.3791/53966 (2016).

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