Journal
/
Biologia do Desenvolvimento
/
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

33,385 Views

•

13:01 min

•

April 10, 2016

DOI:

10.3791/53966-v

DOI:
10.3791/53966-v

•

13:01 min
April 10, 2016

•

23 Views
, , , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Summary

Automatically generated

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

Explore More Videos

Light Sheet Fluorescence MicroscopyZebrafishEye DevelopmentMultiview ReconstructionLow PhototoxicityFast Acquisition3D VolumeBiologia do DesenvolvimentoNeurociênciaAgaroseE3 MediumMS 222Fluorescent Beads

Read Article

Cite this Article

Icha, J., Schmied, C., Sidhaye, J., Tomancak, P., Preibisch, S., Norden, C. Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development. J. Vis. Exp. (110), e53966, doi:10.3791/53966 (2016).

Download .ris file

Copy

Share Video

.

Copy