/
/
تحريض التجريبية المناعة الذاتية التهاب الدماغ في الفئران وتقييم توزيع الأمراض التي تعتمد على خلايا المناعة في الأنسجة المختلفة
A subscription to JoVE is required to view this content. Sign in or start your free trial.
22,944 Views
•
08:47 min
•
May 08, 2016
DOI:
Summary
Automatically generated
This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.
Read Article
Cite this Article
Barthelmes, J., Tafferner, N., Kurz, J., de Bruin, N., Parnham, M. J., Geisslinger, G., Schiffmann, S. Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues. J. Vis. Exp. (111), e53933, doi:10.3791/53933 (2016).
Copy