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Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
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JoVE Journal 免疫学と感染
Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

DOI:
10.3791/53933-v

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08:47 min

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May 08, 2016

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, , , , , ,
1Institute of Clinical Pharmacology,Goethe University Hospital Frankfurt, 2Project Group for Translational Medicine & Pharmacology,Fraunhofer IME

章

  • 00:05標題
  • 01:24Experimental Autoimmune Encephalitis (EAE) Induction and Flow Cytometric Immune Cell Analysis
  • 05:35Results: Representative EAE-induced Spleen, Blood, LN and Spinal Cord Immune Cell and Cytokine mRNA Expression Analyses
  • 07:37Conclusion

概要

自動翻訳

自動翻訳

This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.

タグ

Experimental Autoimmune Encephalomyelitis (EAE)Multiple SclerosisDemyelinationImmune Cell InfiltrationMiceMyelin Oligodendrocyte GlycoproteinComplete Freund’s AdjuvantPertussis ToxinLymph NodeSpinal CordClinical Symptoms

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