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April 19, 2016
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Organ specific sensory neurons are difficult to identify. Fast Blue tracing is used to identify nodose neurons innervating the airways for cell sorting. Sorted nodose neurons are used to extract high quality ribonucleic acid (RNA) for sequencing. Using this protocol, gene expression of airway specific neurons is determined.
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Kaelberer, M. M., Jordt, S. A Method to Target and Isolate Airway-innervating Sensory Neurons in Mice. J. Vis. Exp. (110), e53917, doi:10.3791/53917 (2016).
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