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A Method to Target and Isolate Airway-innervating Sensory Neurons in Mice
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JoVE Revista Neurociencias
A Method to Target and Isolate Airway-innervating Sensory Neurons in Mice

A Method to Target and Isolate Airway-innervating Sensory Neurons in Mice

DOI:
10.3791/53917-v

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07:08 min

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April 19, 2016

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,
1Department of Cellular & Molecular Physiology,Yale University, 2Department of Anesthesiology,Duke University Medical Center

Capítulos

  • 00:05Título
  • 00:38Sensory Ganglia Dissociation
  • 04:10RNA Extraction
  • 04:59Results: Representative FACS and RNA Extraction of Dissociated Ganglia Cells
  • 05:51Conclusion

Summary

Traducción Automática

Traducción Automática

Organ specific sensory neurons are difficult to identify. Fast Blue tracing is used to identify nodose neurons innervating the airways for cell sorting. Sorted nodose neurons are used to extract high quality ribonucleic acid (RNA) for sequencing. Using this protocol, gene expression of airway specific neurons is determined.

Tags

Keywords: Airway-innervating NeuronsNodose NeuronsVagus NervePeripheral NeurobiologySensory NeuronsNeuron IsolationCell DissociationCell SortingFast Blue LabelingDensity Gradient Centrifugation

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