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ゼブラフィッシュ胚からの単一細胞の単離とキャラクタリゼーション
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Characterization of Single Cells from Zebrafish Embryos
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ゼブラフィッシュ胚からの単一細胞の単離とキャラクタリゼーション

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14,212 Views

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09:25 min

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March 12, 2016

DOI:

10.3791/53877-v

DOI:
10.3791/53877-v

•

09:25 min
March 12, 2016

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18 Views
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1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2McAllister Heart Institute,University of North Carolina at Chapel Hill, 3Department of Pathology and Laboratory Medicine,University of North Carolina at Chapel Hill

Summary

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This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

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Single Cell IsolationZebrafish EmbryosMicrofluidic-based Single-cell AnalysisGene ExpressionDevelopmental BiologyCell HeterogeneityCell DissociationCell PreparationChorion RemovalDe-yolkingCell WashingCell Resuspension

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Cite this Article

Samsa, L. A., Fleming, N., Magness, S., Qian, L., Liu, J. Isolation and Characterization of Single Cells from Zebrafish Embryos. J. Vis. Exp. (109), e53877, doi:10.3791/53877 (2016).

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