Journal
/
Entwicklungsbiologie
/
Isolation and Characterization of Single Cells from Zebrafish Embryos
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Isolation and Characterization of Single Cells from Zebrafish Embryos

Isolation and Characterization of Single Cells from Zebrafish Embryos

14,212 Views

•

09:25 min

•

March 12, 2016

DOI:

10.3791/53877-v

DOI:
10.3791/53877-v

•

09:25 min
March 12, 2016

•

18 Views
, , , ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2McAllister Heart Institute,University of North Carolina at Chapel Hill, 3Department of Pathology and Laboratory Medicine,University of North Carolina at Chapel Hill

Summary

Automatically generated

This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

Explore More Videos

Single Cell IsolationZebrafish EmbryosMicrofluidic-based Single-cell AnalysisGene ExpressionEntwicklungsbiologieCell HeterogeneityCell DissociationCell PreparationChorion RemovalDe-yolkingCell WashingCell Resuspension

Read Article

Cite this Article

Samsa, L. A., Fleming, N., Magness, S., Qian, L., Liu, J. Isolation and Characterization of Single Cells from Zebrafish Embryos. J. Vis. Exp. (109), e53877, doi:10.3791/53877 (2016).

Download .ris file

Copy

Share Video

.

Copy