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複合光遺伝学および凍結破砕レプリカ免疫標識をマウス扁桃体におけるグルタミン酸受容体の入力固有の配置を検討すること
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala
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複合光遺伝学および凍結破砕レプリカ免疫標識をマウス扁桃体におけるグルタミン酸受容体の入力固有の配置を検討すること

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10,758 Views

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09:49 min

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April 15, 2016

DOI:

10.3791/53853-v

DOI:
10.3791/53853-v

•

09:49 min
April 15, 2016

•

2 Views
, , , , ,
1Department of Pharmacology,Medical University of Innsbruck, 2Hertie Institute for Clinical Brain Research and Centre for Integrative Neuroscience,University of Tübingen

Summary

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This article illustrates how the expression of neurotransmitter receptors can be quantified and the pattern analyzed at synapses with identified pre and postsynaptic elements using a combination of viral transduction of optogenetic tools and the freeze-fracture replica immunolabeling technique.

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Cite this Article

Schönherr, S., Seewald, A., Kasugai, Y., Bosch, D., Ehrlich, I., Ferraguti, F. Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala. J. Vis. Exp. (110), e53853, doi:10.3791/53853 (2016).

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