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Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

10,795 Views

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09:49 min

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April 15, 2016

DOI:

10.3791/53853-v

DOI:
10.3791/53853-v

•

09:49 min
April 15, 2016

•

2 Views
, , , , ,
1Department of Pharmacology,Medical University of Innsbruck, 2Hertie Institute for Clinical Brain Research and Centre for Integrative Neuroscience,University of Tübingen

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This article illustrates how the expression of neurotransmitter receptors can be quantified and the pattern analyzed at synapses with identified pre and postsynaptic elements using a combination of viral transduction of optogenetic tools and the freeze-fracture replica immunolabeling technique.

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Schönherr, S., Seewald, A., Kasugai, Y., Bosch, D., Ehrlich, I., Ferraguti, F. Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala. J. Vis. Exp. (110), e53853, doi:10.3791/53853 (2016).

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