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Enzimatik, kimyasal (Metilasyon) ve Fiziksel (Kütle Spektrometre, Nükleer Manyetik Rezonans) Teknikleri Kullanarak Bitki Duvar heteroksilanlar sıralanması
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Chemistry
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JoVE Journal Chemistry
Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
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Enzimatik, kimyasal (Metilasyon) ve Fiziksel (Kütle Spektrometre, Nükleer Manyetik Rezonans) Teknikleri Kullanarak Bitki Duvar heteroksilanlar sıralanması

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7,548 Views

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11:49 min

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March 24, 2016

DOI:

10.3791/53748-v

DOI:
10.3791/53748-v

•

11:49 min
March 24, 2016

•

2 Views
, ,
1ARC Centre of Excellence in Plant Cell Walls, School of BioSciences,University of Melbourne

Summary

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This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).

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Cite this Article

Ratnayake, S., Ford, K., Bacic, A. Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques. J. Vis. Exp. (109), e53748, doi:10.3791/53748 (2016).

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