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Le séquençage de l'usine mur hétéroxylanes Utilisation enzymatique, chimique (méthylation) et physiques (spectrométrie de masse, résonance magnétique nucléaire) Techniques
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Chemistry
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JoVE Journal Chemistry
Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
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Le séquençage de l'usine mur hétéroxylanes Utilisation enzymatique, chimique (méthylation) et physiques (spectrométrie de masse, résonance magnétique nucléaire) Techniques

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7,548 Views

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11:49 min

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March 24, 2016

DOI:

10.3791/53748-v

DOI:
10.3791/53748-v

•

11:49 min
March 24, 2016

•

2 Views
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1ARC Centre of Excellence in Plant Cell Walls, School of BioSciences,University of Melbourne

Summary

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This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).

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Ratnayake, S., Ford, K., Bacic, A. Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques. J. Vis. Exp. (109), e53748, doi:10.3791/53748 (2016).

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