Journal
/
Developmental Biology
/
Basicおよび前臨床研究のための密閉型細胞培養系でヒト多能性および神経幹細胞を培養します
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
Please note that all translations are automatically generated. Click here for the English version.

Basicおよび前臨床研究のための密閉型細胞培養系でヒト多能性および神経幹細胞を培養します

日本語

Automatically Generated

9,943 Views

•

08:05 min

•

June 10, 2016

DOI:

10.3791/53685-v

DOI:
10.3791/53685-v

•

08:05 min
June 10, 2016

•

3 Views
, , , , ,
1National Human Neural Stem Cell Resource,Childrens Hospital of Orange County Research Institute

Summary

Automatically generated

Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.

Explore More Videos

Human Pluripotent Stem CellsNeural Stem CellsEnclosed Cell Culture SystemBasic ResearchPreclinical ResearchCell Culture ParametersMultipotent Stem CellsPluripotent Stem CellsOxygenCarbon DioxideTemperatureIncubatorProcess ChamberBuffer ChamberLaminar HoodSterilityCell Culture Procedure

Read Article

Cite this Article

Stover, A. E., Herculian, S., Banuelos, M. G., Navarro, S. L., Jenkins, M. P., Schwartz, P. H. Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research. J. Vis. Exp. (112), e53685, doi:10.3791/53685 (2016).

Download .ris file

Copy

Share Video

.

Copy