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Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
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発生生物学
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JoVE Journal 発生生物学
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

9,875 Views

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08:05 min

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June 10, 2016

DOI:

10.3791/53685-v

DOI:
10.3791/53685-v

•

08:05 min
June 10, 2016

•

3 Views
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1National Human Neural Stem Cell Resource,Childrens Hospital of Orange County Research Institute

概要

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Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.

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Cite this Article

Stover, A. E., Herculian, S., Banuelos, M. G., Navarro, S. L., Jenkins, M. P., Schwartz, P. H. Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research. J. Vis. Exp. (112), e53685, doi:10.3791/53685 (2016).

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