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Entrega mediada por fago de sRNA alvejado Constrói para bater para baixo Expressão Gênica em<em> E. coli</em
JoVE Journal
Biology
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JoVE Journal Biology
Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Entrega mediada por fago de sRNA alvejado Constrói para bater para baixo Expressão Gênica em<em> E. coli</em

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12,342 Views

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08:25 min

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March 20, 2016

DOI:

10.3791/53618-v

DOI:
10.3791/53618-v

•

08:25 min
March 20, 2016

•

7 Views
, , ,
1U1001,Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes

Summary

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We describe a method to knock down gene expression in a growing population of E. coli cells using sequence-targeted sRNA expression cassettes delivered by an M13 phagemid vector.

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Cite this Article

Bernheim, A. G., Libis, V. K., Lindner, A. B., Wintermute, E. H. Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli. J. Vis. Exp. (109), e53618, doi:10.3791/53618 (2016).

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