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Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
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March 20, 2016
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We describe a method to knock down gene expression in a growing population of E. coli cells using sequence-targeted sRNA expression cassettes delivered by an M13 phagemid vector.
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Bernheim, A. G., Libis, V. K., Lindner, A. B., Wintermute, E. H. Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli. J. Vis. Exp. (109), e53618, doi:10.3791/53618 (2016).
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