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Multifunzionale, Metodo Micropipetta-based per la Costituzione e la stimolazione della batteriche meccanosensibili Ion Canali in Droplet Interface bilayers
JoVE Journal
Biology
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JoVE Journal Biology
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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Multifunzionale, Metodo Micropipetta-based per la Costituzione e la stimolazione della batteriche meccanosensibili Ion Canali in Droplet Interface bilayers

italiano

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10,593 Views

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09:54 min

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November 19, 2015

DOI:

10.3791/53362-v

DOI:
10.3791/53362-v

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09:54 min
November 19, 2015

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4 Views
, , , , , ,
1Department of Mechanical Engineering,Virginia Polytechnic Institute and State University, 2School of Biomedical Engineering and Sciences,Virginia Polytechnic Institute and State University, 3Department of Biology,University of Maryland, 4College of Engineering,University of Georgia, 5Department of Engineering Sciences and Mechanics,Virginia Polytechnic Institute and State University

Summary

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Canali meccanosensibili batteriche possono essere usati come trasduttori mechanoelectrical nei dispositivi biomolecolari. Bistrati interfaccia Droplet (DIB), cellula di ispirazione blocchi di costruzione di tali dispositivi, rappresentano nuove piattaforme per incorporare e stimolare canali meccanosensibili. Qui, dimostriamo un nuovo metodo basato micropipetta-di formare DIB, permettendo lo studio di canali meccanosensibili sotto stimolazione meccanica.

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Najem, J. S., Dunlap, M. D., Yasmann, A., Freeman, E. C., Grant, J. W., Sukharev, S., Leo, D. J. Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers. J. Vis. Exp. (105), e53362, doi:10.3791/53362 (2015).

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