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Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
JoVE Journal
生物学
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JoVE Journal 生物学
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

10,593 Views

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09:54 min

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November 19, 2015

DOI:

10.3791/53362-v

DOI:
10.3791/53362-v

•

09:54 min
November 19, 2015

•

4 Views
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1機械工学科,Virginia Polytechnic Institute and State University, 2School of Biomedical Engineering and Sciences,Virginia Polytechnic Institute and State University, 3生物学科,University of Maryland, 4College of Engineering,University of Georgia, 5Department of Engineering Sciences and Mechanics,Virginia Polytechnic Institute and State University

概要

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Bacterial mechanosensitive channels can be used as mechanoelectrical transducers in biomolecular devices. Droplet interface bilayers (DIBs), cell-inspired building blocks to such devices, represent new platforms to incorporate and stimulate mechanosensitive channels. Here, we demonstrate a new micropipette-based method of forming DIBs, allowing the study of mechanosensitive channels under mechanical stimulation.

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Najem, J. S., Dunlap, M. D., Yasmann, A., Freeman, E. C., Grant, J. W., Sukharev, S., Leo, D. J. Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers. J. Vis. Exp. (105), e53362, doi:10.3791/53362 (2015).

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