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Quantificazione tridimensionale di spine dendritiche da piramidale neuroni derivate da cellule staminali umane pluripotenti indotte
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10:18 min
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October 10, 2015
DOI:
1Human Genetics and Cognitive Functions,Institut Pasteur, 2CNRS URA 2182 ‘Genes, synapses and cognition’,Institut Pasteur, 3Human Genetics and Cognitive Functions,Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d’ Imagerie Dynamique, Imagopole, CiTech,Institut Pasteur, 5Neuroplasticity and Therapeutics,CECS, I-STEM, AFM, Evry
Summary
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Spine dendritiche di neuroni piramidali sono i siti di maggior sinapsi eccitatorie in mammiferi corteccia cerebrale. Il metodo descrive un'analisi quantitativa 3D di morfologie della colonna vertebrale nei neuroni piramidali glutamatergici corticali umane derivate da cellule staminali pluripotenti indotte.
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Cite this Article
Gouder, L., Tinevez, J., Goubran-Botros, H., Benchoua, A., Bourgeron, T., Cloëz-Tayarani, I. Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (104), e53197, doi:10.3791/53197 (2015).
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