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Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
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Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
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10:18 min
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October 10, 2015
DOI:
1Human Genetics and Cognitive Functions,Pasteur Enstitüsü, 2CNRS URA 2182 ‘Genes, synapses and cognition’,Pasteur Enstitüsü, 3Human Genetics and Cognitive Functions,Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d’ Imagerie Dynamique, Imagopole, CiTech,Pasteur Enstitüsü, 5Neuroplasticity and Therapeutics,CECS, I-STEM, AFM, Evry
Özet
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Dendritic spines of pyramidal neurons are the sites of most excitatory synapses in mammalian brain cortex. This method describes a 3D quantitative analysis of spine morphologies in human cortical pyramidal glutamatergic neurons derived from induced pluripotent stem cells.
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Gouder, L., Tinevez, J., Goubran-Botros, H., Benchoua, A., Bourgeron, T., Cloëz-Tayarani, I. Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (104), e53197, doi:10.3791/53197 (2015).
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