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1Program in Genetics & Genome Biology,The Hospital for Sick Children, 2Department of Molecular Genetics,The University of Toronto, 3Program in Genomics of Differentiation,Eunice Kennedy Shriver National Institute of Child Health and Human Development, 4Departments of Pediatrics and Neurology,University of Michigan
Chapters
- 00:05Title
- 01:14Preparation of Agar Injection Plates and Evans Blue Dye Injection Mix
- 02:42Evans Blue Dye Injection Preparation
- 03:54Pericardial Injection and Uptake of Evans Blue Dye in Zebrafish Larvae
- 05:29Results: Skeletal Muscle Analysis in Zebrafish Larvae Using Evans Blue Dye
- 06:14Conclusion
In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae. This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.
