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性T细胞Transinfection从树突状细胞捕捉细菌
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Immunology and Infection
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JoVE Journal Immunology and Infection
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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性T细胞Transinfection从树突状细胞捕捉细菌

DOI:
10.3791/52976-v

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11:39 min

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January 13, 2016

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, , , ,
1Cellular and Molecular Biology, Spanish National Research Council (CSIC),Spanish National Biotechnology Centre (CNB-CSIC), 2Hospital de Santa Cristina,Healthcare Research Institute Princesa Hospital (IIS-Princesa)

Chapters

  • 00:05Title
  • 01:28Maturation of Mouse Bone Marrow-derived DCs, Antigen Loading and Infection
  • 04:18T Cell Transinfection Measurement by Gentamicin Protection Assay
  • 05:54Re-isolation of CD4+ T Cells
  • 07:13Determining Transinfection Rate
  • 09:05T Cell Transinfection with Salmonella Enteritidis Quantified by Gentamicin
  • 10:26Conclusion

Summary

Automatic Translation

Automatic Translation

这里的协议，提出通过该期间抗原呈递发生经由transinfection从预感染树突状细胞（DC）的CD4 + T细胞以测量细菌捕获。我们说明如何执行必要的步骤：原代细胞中，DC，DC / T细胞共轭形成感染和细菌的T细胞转染的测量隔离。

Tags

T CellsBacteriaTransinfectionDendritic CellsListeria MonocytogenesSalmonella EnteritidisAntigen PresentationPhagocytesInnate ImmunityAdaptive ImmunityBone Marrow Derived Dendritic CellsMHC IIFlow CytometryOT-II OVAp

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