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الجيل القادم التسلسل للكشف عن المنحى الطفرات في الأورام الصلبة والسائلة
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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الجيل القادم التسلسل للكشف عن المنحى الطفرات في الأورام الصلبة والسائلة

العربية

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23,815 Views

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11:15 min

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September 20, 2016

DOI:

10.3791/52758-v

DOI:
10.3791/52758-v

•

11:15 min
September 20, 2016

•

44 Views
, , , , , , , , , , , ,
1Department of Pathology and Laboratory Medicine,Perelman School of Medicine, University of Pennsylvania, 2Division of Hematology/Oncology, Department of Medicine,Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center

Summary

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This manuscript describes clinical protocols for two next-generation sequencing panels. One panel interrogates hematologic malignancies while the other panel targets genes commonly mutated in solid tumors. Molecular classification of driver mutations in human malignancies offers valuable prognostic and predictive information.

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Next Generation SequencingTargeted Amplicon AssayActionable MutationsSolid TumorsLiquid TumorsTherapeutic UseDiagnosticPrognosticResponse To Targeted TherapyGene MutationsMedical OncologyGenomic DNAAmplicon LibraryOligo HybridizationFilter Plate UnitExtension Ligation

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Fox, A. J., Hiemenz, M. C., Lieberman, D. B., Sukhadia, S., Li, B., Grubb, J., Candrea, P., Ganapathy, K., Zhao, J., Roth, D., Alley, E., Loren, A., Morrissette, J. J. D. Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors. J. Vis. Exp. (115), e52758, doi:10.3791/52758 (2016).

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