Journal
/
癌症研究
/
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
JoVE 杂志
癌症研究
This content is Free Access.
JoVE 杂志 癌症研究
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

23,816 Views

•

11:15 min

•

September 20, 2016

DOI:

10.3791/52758-v

DOI:
10.3791/52758-v

•

11:15 min
September 20, 2016

•

44 Views
, , , , , , , , , , , ,
1Department of Pathology and Laboratory Medicine,Perelman School of Medicine, University of Pennsylvania, 2Division of Hematology/Oncology, Department of Medicine,Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center

Summary

Automatically generated

This manuscript describes clinical protocols for two next-generation sequencing panels. One panel interrogates hematologic malignancies while the other panel targets genes commonly mutated in solid tumors. Molecular classification of driver mutations in human malignancies offers valuable prognostic and predictive information.

Explore More Videos

Next Generation SequencingTargeted Amplicon AssayActionable MutationsSolid TumorsLiquid TumorsTherapeutic UseDiagnosticPrognosticResponse To Targeted TherapyGene MutationsMedical OncologyGenomic DNAAmplicon LibraryOligo HybridizationFilter Plate UnitExtension Ligation

Read Article

Cite this Article

Fox, A. J., Hiemenz, M. C., Lieberman, D. B., Sukhadia, S., Li, B., Grubb, J., Candrea, P., Ganapathy, K., Zhao, J., Roth, D., Alley, E., Loren, A., Morrissette, J. J. D. Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors. J. Vis. Exp. (115), e52758, doi:10.3791/52758 (2016).

Download .ris file

Copy

Share Video

.

Copy