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Cancer Research

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JoVE Journal Cancer Research
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
 

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Article DOI: 10.3791/52758-v • 11:15 min • September 20th, 2016
September 20th, 2016 •
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1Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, 2Division of Hematology/Oncology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center

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This manuscript describes clinical protocols for two next-generation sequencing panels. One panel interrogates hematologic malignancies while the other panel targets genes commonly mutated in solid tumors. Molecular classification of driver mutations in human malignancies offers valuable prognostic and predictive information.

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Next Generation Sequencing Detect Actionable Mutations Solid Tumors Liquid Tumors Amplicon Assay Therapeutic Use Diagnostic Prognostic Targeted Therapy Complex Techniques Visual Demonstration Advancing Medical Oncology Cancer Treatments CPD Technologists Genomic DNA Extraction Fluorometer Amplicon Library Hyb Plate
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