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Escalável placa de 96 poços Baseado iPSC Cultura e Produção Usando um sistema de manipulação robótica Líquido
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Escalável placa de 96 poços Baseado iPSC Cultura e Produção Usando um sistema de manipulação robótica Líquido

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08:00 min

May 14, 2015

DOI:

08:00 min
May 14, 2015

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Summary

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The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.

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