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Scalable plaque de 96 puits Basé iPSC Culture et production en utilisant un système robotique Liquid Handling
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May 14, 2015
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Summary
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The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
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Conway, M. K., Gerger, M. J., Balay, E. E., O'Connell, R., Hanson, S., Daily, N. J., Wakatsuki, T. Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System. J. Vis. Exp. (99), e52755, doi:10.3791/52755 (2015).
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