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Immunology and Infection
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Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

English

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35,607 Views

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08:21 min

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April 29, 2015

DOI:

10.3791/52749-v

DOI:
10.3791/52749-v

•

08:21 min
April 29, 2015

•

36 Views
, ,
1Centre de recherche du Centre hospitalier de l’Université de Montréal,Institut du cancer de Montréal, 2Département de Médecine,Université de Montréal

Summary

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We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.

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Layoun, A., Samba, M., Santos, M. M. Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation. J. Vis. Exp. (98), e52749, doi:10.3791/52749 (2015).

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