ジャーナル
/
免疫学と感染
/
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

35,607 Views

•

08:21 min

•

April 29, 2015

DOI:

10.3791/52749-v

DOI:
10.3791/52749-v

•

08:21 min
April 29, 2015

•

36 Views
, ,
1Centre de recherche du Centre hospitalier de l’Université de Montréal,Institut du cancer de Montréal, 2Département de Médecine,Université de Montréal

概要

Automatically generated

We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.

Explore More Videos

Peritoneal MacrophagesToll-like ReceptorsGene ExpressionInflammationMonocyte MigrationBrewer’s Thioglycollate MediumTLR LigandsImmunological StudiesMetabolic Studies

Read Article

Cite this Article

Layoun, A., Samba, M., Santos, M. M. Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation. J. Vis. Exp. (98), e52749, doi:10.3791/52749 (2015).

Download .ris file

Copy

Share Video

.

Copy