/
/
Bir Enzimatik Yöntem Kaymaklı Kemik İliği Örneklerinden Mezenkimal Kök Hücreler Kurtarma
A subscription to JoVE is required to view this content. Sign in or start your free trial.
9,085 Views
•
08:58 min
•
April 12, 2015
DOI:
Summary
Automatically generated
Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.
Read Article
Cite this Article
Schlaefli, P., Bertolo, A., Malonzo, C., Poetzel, T., Baur, M., Steffen, F., Stoyanov, J. An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples. J. Vis. Exp. (98), e52694, doi:10.3791/52694 (2015).
Copy