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على الطريقة الأنزيمية لانقاذ الوسيطة الخلايا الجذعية من عينات متخدر نخاع العظم
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

على الطريقة الأنزيمية لانقاذ الوسيطة الخلايا الجذعية من عينات متخدر نخاع العظم

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08:58 min

April 12, 2015

DOI:

08:58 min
April 12, 2015

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Summary

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Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.

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