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Time-lapse aydınlık stereomikroskobi ile Zebra balığı Larva Doku Onarım yakalama
JoVE Journal
Developmental Biology
Author Produced
This content is Free Access.
JoVE Journal Developmental Biology
Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Time-lapse aydınlık stereomikroskobi ile Zebra balığı Larva Doku Onarım yakalama

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14:29 min

January 31, 2015

DOI:

14:29 min
January 31, 2015

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Summary

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We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.

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