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التقاط إصلاح الأنسجة في الزرد اليرقات مع الوقت الفاصل بين Brightfield Stereomicroscopy
JoVE Journal
Developmental Biology
Author Produced
This content is Free Access.
JoVE Journal Developmental Biology
Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

التقاط إصلاح الأنسجة في الزرد اليرقات مع الوقت الفاصل بين Brightfield Stereomicroscopy

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14:29 min

January 31, 2015

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14:29 min
January 31, 2015

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Summary

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We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.

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