Journal
/
Immunology and Infection
/
İnsan İmmün Yetmezlik Virüs çoğaltma Fitness belirlenmesi için İkili Büyüme Rekabet Analizi
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
Please note that all translations are automatically generated. Click here for the English version.

İnsan İmmün Yetmezlik Virüs çoğaltma Fitness belirlenmesi için İkili Büyüme Rekabet Analizi

Türkçe

Automatically Generated

10,913 Views

•

11:19 min

•

May 04, 2015

DOI:

10.3791/52610-v

DOI:
10.3791/52610-v

•

11:19 min
May 04, 2015

•

2 Views
, , , , , , , , , , ,
1Department of Microbiology,University of Washington, 2Departments of Medicine and Laboratory Medicine,University of Washington, 3U.S Military HIV Research Program,Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation

Summary

Automatically generated

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

Explore More Videos

HIV-1Viral Replication FitnessIn Vitro Fitness AssayGrowth Competition AssayMultiplicity Of Infection (MOI)Viral DetectionRelative Fitness Calculation

Read Article

Cite this Article

Manocheewa, S., Lanxon-Cookson, E. C., Liu, Y., Swain, J. V., McClure, J., Rao, U., Maust, B., Deng, W., Sunshine, J. E., Kim, M., Rolland, M., Mullins, J. I. Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses. J. Vis. Exp. (99), e52610, doi:10.3791/52610 (2015).

Download .ris file

Copy

Share Video

.

Copy